KHDRBS1 Antibody

ProSci
Product Code: PSI-22-007
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-007-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using KHDRBS1 antibody (22-007) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat brain using KHDRBS1 antibody (22-007) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate cancer using KHDRBS1 antibody (22-007) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using KHDRBS1 antibody (22-007) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using KHDRBS1 antibody (22-007) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using KHDRBS1 antibody (22-007) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using KHDRBS1 antibody (22-007) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using KHDRBS1 antibody (22-007) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using KHDRBS1 antibody (22-007) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using KHDRBS1 antibody (22-007) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using KHDRBS1 antibody (22-007) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using KHDRBS1 antibody (22-007) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
KHDRBS1, FLJ34027, Sam68, p62, p68
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human KHDRBS1 (NP_006550.1).
NCBI Gene ID #:
10657
NCBI Official Name:
KH domain containing, RNA binding, signal transduction associated 1
NCBI Official Symbol:
KHDRBS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q07666
User NOte:
Optimal dilutions for each application to be determined by the researcher.