CRABP2 Antibody

ProSci
Product Code: PSI-22-012
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-012-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CRABP2 antibody (22-012) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat brain using CRABP2 antibody (22-012) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using CRABP2 antibody (22-012). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CRABP2 antibody (22-012) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CRABP2 antibody (22-012) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CRABP2 antibody (22-012). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CRABP2, CRABP-II, RBP6
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:20 - 1:100
Background:
This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-94 of human CRABP2 (NP_001869.1).
NCBI Gene ID #:
1382
NCBI Official Name:
cellular retinoic acid binding protein 2
NCBI Official Symbol:
CRABP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 15kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P29373
User NOte:
Optimal dilutions for each application to be determined by the researcher.