SMARCC1 Antibody, KO Validated

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ProSci
Product Code: PSI-22-018
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-22-018-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SMARCC1 antibody (22-018) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
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Western blot analysis of extracts from normal (control) and SMARCC1 knockout (KO) HeLa cells, using SMARCC1 antibody (22-018) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
3 / 4
Immunohistochemistry of paraffin-embedded human kidney cancer using SMARCC1 antibody (22-018) at dilution of 1:200 (40x lens).
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug SMARCC1 antibody (22-018). Western blot was performed from the immunoprecipitate using SMARCC1 antibody (22-018) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using SMARCC1 antibody (22-018) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
Western blot analysis of extracts from normal (control) and SMARCC1 knockout (KO) HeLa cells, using SMARCC1 antibody (22-018) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human kidney cancer using SMARCC1 antibody (22-018) at dilution of 1:200 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug SMARCC1 antibody (22-018). Western blot was performed from the immunoprecipitate using SMARCC1 antibody (22-018) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
SMARCC1, Rsc8, SRG3, SWI3, BAF155, CRACC1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1 (NP_003065.3).
NCBI Gene ID #:
6599
NCBI Official Name:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
NCBI Official Symbol:
SMARCC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 155kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q92922
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.