SETDB1 Antibody

ProSci
Product Code: PSI-22-030
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-030-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SETDB1 antibody (22-030) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 2
Immunohistochemistry of paraffin-embedded rat brain using SETDB1 antibody (22-030) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using SETDB1 antibody (22-030) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using SETDB1 antibody (22-030) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
SETDB1, H3-K9-HMTase4, ESET, KMT1E, H3-K9-HMTase 4, KG1T, SET domain, bifurcated 1, SET domain bifurcated 1, KIAA0067, Lysine N-methyltransferase 1E
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-397 of human SETDB1 (NP_001230420.1).
NCBI Gene ID #:
9869
NCBI Official Name:
SET domain, bifurcated 1
NCBI Official Symbol:
SETDB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 210kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q15047
User NOte:
Optimal dilutions for each application to be determined by the researcher.