SF3B3 Antibody

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ProSci
Product Code: PSI-22-031
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-031-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SF3B3 antibody (22-031) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.
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Immunohistochemistry of paraffin-embedded rat brain using SF3B3 antibody (22-031) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using SF3B3 antibody (22-031) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using SF3B3 antibody (22-031) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using SF3B3 antibody (22-031) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.
Immunohistochemistry of paraffin-embedded rat brain using SF3B3 antibody (22-031) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using SF3B3 antibody (22-031) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SF3B3 antibody (22-031) at dilution of 1:200 (40x lens).

Documents

Further Information

Additional Names:
SF3B3, KIAA0017, RSE1, SAP130, SF3b130, STAF130
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 948-1217 of human SF3B3 (NP_036558.3).
NCBI Gene ID #:
23450
NCBI Official Name:
splicing factor 3b, subunit 3, 130kDa
NCBI Official Symbol:
SF3B3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 136kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
Q15393
User NOte:
Optimal dilutions for each application to be determined by the researcher.