HMGN1 Antibody

ProSci
Product Code: PSI-22-035
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-035-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HMGN1 antibody (22-035) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human stomach using HMGN1 antibody (22-035) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using HMGN1 antibody (22-035) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using HMGN1 antibody (22-035) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HMGN1 antibody (22-035) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using HMGN1 antibody (22-035) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HMGN1 antibody (22-035) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HMGN1 antibody (22-035) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HMGN1, FLJ27265, FLJ31471, HMG14, MGC104230, MGC117425
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGN1 (NP_004956.5).
NCBI Gene ID #:
3150
NCBI Official Name:
high mobility group nucleosome binding domain 1
NCBI Official Symbol:
HMGN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P05114
User NOte:
Optimal dilutions for each application to be determined by the researcher.