GSK3B Antibody, KO Validated

ProSci
Product Code: PSI-22-038
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-038-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using GSK3B antibody (22-038) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Western blot analysis of extracts from normal (control) and GSK3B knockout (KO) HeLa cells, using GSK3B antibody (22-038) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
3 / 4
Immunofluorescence analysis of HeLa cells using GSK3B antibody (22-038) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug GSK3B antibody (22-038). Western blot was performed from the immunoprecipitate using GSK3B antibody (22-038) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using GSK3B antibody (22-038) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts from normal (control) and GSK3B knockout (KO) HeLa cells, using GSK3B antibody (22-038) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using GSK3B antibody (22-038) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug GSK3B antibody (22-038). Western blot was performed from the immunoprecipitate using GSK3B antibody (22-038) at a dilition of 1:1000.

Further Information

Additional Names:
GSK3B, GSK-3 beta, GSK3 beta, GSK3beta, GSK3beta isoform, TPKI, GSK 3beta, Tau protein kinase I, ZGSK-3beta
Application Note:
WB: 1:500 - 1:1000

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GSK3B (NP_001139628.1).
NCBI Gene ID #:
2932
NCBI Official Name:
glycogen synthase kinase 3 beta
NCBI Official Symbol:
GSK3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P49841
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.