HAT1 Antibody

ProSci
Product Code: PSI-22-046
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-046-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HAT1 antibody (22-046) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse osteosarcoma using HAT1 antibody (22-046) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U2OS cells using HAT1 antibody (22-046). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HAT1 antibody (22-046) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse osteosarcoma using HAT1 antibody (22-046) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using HAT1 antibody (22-046). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1, HAT1, KAT1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

ChIP: 1:50 - 1:200
Background:
The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 245-419 of human HAT1 (NP_003633.1).
NCBI Gene ID #:
8520
NCBI Official Name:
Histone acetyltransferase type B catalytic subunit
NCBI Official Symbol:
HAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
O14929
User NOte:
Optimal dilutions for each application to be determined by the researcher.