SET Antibody

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ProSci
Product Code: PSI-22-055
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-055-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SET antibody (22-055) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded mouse kidney using SET antibody (22-055) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using SET antibody (22-055) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using SET antibody (22-055) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using SET antibody (22-055) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using SET antibody (22-055) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SET antibody (22-055) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse kidney using SET antibody (22-055) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SET antibody (22-055) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using SET antibody (22-055) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using SET antibody (22-055) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using SET antibody (22-055) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
SET, SET translocation (myeloid leukemia-associated), 2PP2A, I2PP2A, PHAPII, TAF-IBETA, SET, translocation, IGAAD, inhibitor-2 of protein phosphatase-2A, Inhibitor of GZMA-activated DNAse, TAF-I, IPP2A2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human SET (NP_003002.2).
NCBI Gene ID #:
6418
NCBI Official Name:
SET nuclear oncogene
NCBI Official Symbol:
SET
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
Q01105
User NOte:
Optimal dilutions for each application to be determined by the researcher.