FLOT1 Antibody

ProSci
Product Code: PSI-22-060
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-060-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using FLOT1 antibody (22-060) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunofluorescence analysis of U2OS cells using FLOT1 antibody (22-060). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using FLOT1 antibody (22-060) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using FLOT1 antibody (22-060). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae,
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 128-427 of human FLOT1 (NP_005794.1).
NCBI Gene ID #:
10211
NCBI Official Name:
flotillin 1
NCBI Official Symbol:
FLOT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
O75955
User NOte:
Optimal dilutions for each application to be determined by the researcher.