ESD Antibody

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ProSci
Product Code: PSI-22-066
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-066-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ESD antibody (22-066) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded Human trachea using ESD antibody (22-066) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using ESD antibody (22-066) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using ESD antibody (22-066) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded Human trachea using ESD antibody (22-066) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ESD antibody (22-066) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
S-formylglutathione hydrolase, FGH, Esterase D, Methylumbelliferyl-acetate deacetylase, ESD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human ESD (NP_001975.1).
NCBI Gene ID #:
2098
NCBI Official Name:
S-formylglutathione hydrolase
NCBI Official Symbol:
ESD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P10768
User NOte:
Optimal dilutions for each application to be determined by the researcher.