FDFT1 Antibody

ProSci
Product Code: PSI-22-067
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-067-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using FDFT1 antibody (22-067) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
2 / 4
Immunohistochemistry of paraffin-embedded mouse testis using FDFT1 antibody (22-067) at dilution of 1:100 (20x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using FDFT1 antibody (22-067) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of NIH-3T3 cells using FDFT1 antibody (22-067) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using FDFT1 antibody (22-067) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse testis using FDFT1 antibody (22-067) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse brain using FDFT1 antibody (22-067) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using FDFT1 antibody (22-067) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
FDFT1, DGPT, ERG9, SQS, SS
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human FDFT1 (NP_004453.3).
NCBI Gene ID #:
2222
NCBI Official Name:
farnesyl-diphosphate farnesyltransferase 1
NCBI Official Symbol:
FDFT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience
Swissprot #:
P37268
User NOte:
Optimal dilutions for each application to be determined by the researcher.