OAT Antibody

ProSci
Product Code: PSI-22-072
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-072-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using OAT antibody (22-072) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug OAT antibody (22-072). Western blot was performed from the immunoprecipitate using OAT antibody (22-072) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using OAT antibody (22-072) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug OAT antibody (22-072). Western blot was performed from the immunoprecipitate using OAT antibody (22-072) at a dilition of 1:1000.

Further Information

Additional Names:
OAT, DKFZp781A11155, HOGA, OKT, GACR, OATASE
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Background:
This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1).
NCBI Gene ID #:
4942
NCBI Official Name:
ornithine aminotransferase
NCBI Official Symbol:
OAT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P04181
User NOte:
Optimal dilutions for each application to be determined by the researcher.