ALKBH1 Antibody

ProSci
Product Code: PSI-22-075
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-075-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ALKBH1 antibody (22-075) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug ALKBH1 antibody (22-075). Western blot was performed from the immunoprecipitate using ALKBH1 antibody (22-075) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using ALKBH1 antibody (22-075) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug ALKBH1 antibody (22-075). Western blot was performed from the immunoprecipitate using ALKBH1 antibody (22-075) at a dilition of 1:1000.

Further Information

Additional Names:
ABH, ABH1, ALKBH, alkB, hABH, nucleic acid dioxygenase ALKBH1, DNA 6mA demethylase, DNA N6-methyl adenine demethylase, DNA demethylase ALKBH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, alkB, alkylation repair homolog 1, alkylated DNA repair protein alkB homolog 1, alkylation repair, alkB homolog, alpha-ketoglutarate-dependent dioxygenase ABH1, tRNA N1-methyl adenine demethylase
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:200
Background:
This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2).
NCBI Gene ID #:
8846
NCBI Official Name:
alkB homolog 1, histone H2A dioxygenase
NCBI Official Symbol:
ALKBH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13686
User NOte:
Optimal dilutions for each application to be determined by the researcher.