PHLDA2 Antibody

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ProSci
Product Code: PSI-22-077
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-077-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PHLDA2 antibody (22-077) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunofluorescence analysis of HeLa cells using PHLDA2 antibody (22-077). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PHLDA2 antibody (22-077) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using PHLDA2 antibody (22-077). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
PHLDA2, pleckstrin homology-like domain, family A, member 2, TSSC3, IPL, BRW1C, BWR1C, HLDA2, PHLDA2, tumor suppressing subtransferable candidate 3, tumor-supressing STF cDNA 3, imprinted in placenta and liver, p17-Beckwith-Wiedemann region 1C, pleckstrin homology-like domain family A member 2, tumor suppressing subchromosomal transferable fragment cDNA 3
Application Note:
WB: 1:500 - 1:2000

IF: 1:20 - 1:100
Background:
This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human PHLDA2 (NP_003302.1).
NCBI Gene ID #:
7262
NCBI Official Name:
pleckstrin homology-like domain, family A, member 2
NCBI Official Symbol:
PHLDA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
Q53GA4
User NOte:
Optimal dilutions for each application to be determined by the researcher.