GAB1 Antibody

ProSci
Product Code: PSI-22-081
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-081-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GAB1 antibody (22-081) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 80s.
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Immunohistochemistry of paraffin-embedded rat brain using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spinal cord using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using GAB1 antibody (22-081). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GAB1 antibody (22-081) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 80s.
Immunohistochemistry of paraffin-embedded rat brain using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using GAB1 antibody (22-081) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using GAB1 antibody (22-081). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ADDA, Growth factor receptor bound protein 2-associated protein 1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:20 - 1:100
Background:
The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 485-724 of human GAB1 (NP_997006.1).
NCBI Gene ID #:
2549
NCBI Official Name:
GRB2-associated binding protein 1
NCBI Official Symbol:
GAB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 76kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
Q13480
User NOte:
Optimal dilutions for each application to be determined by the researcher.