COG2 Antibody

ProSci
Product Code: PSI-22-084
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-084-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using COG2 antibody (22-084) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 80s.
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Immunohistochemistry of paraffin-embedded rat brain using COG2 antibody (22-084) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using COG2 antibody (22-084) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U-2 OS cells using COG2 Polyclonal Antibody (22-084) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using COG2 antibody (22-084) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 80s.
Immunohistochemistry of paraffin-embedded rat brain using COG2 antibody (22-084) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using COG2 antibody (22-084) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using COG2 Polyclonal Antibody (22-084) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
COG2, LDLC
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi complex. The encoded protein specifically interacts with the USO1 vesicle docking protein and may be necessary for normal Golgi ribbon formation and trafficking of Golgi enzymes. Mutations of this gene are associated with abnormal glycosylation within the Golgi apparatus. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human COG2 (NP_031383.1).
NCBI Gene ID #:
22796
NCBI Official Name:
component of oligomeric golgi complex 2
NCBI Official Symbol:
COG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 88kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q14746
User NOte:
Optimal dilutions for each application to be determined by the researcher.