SERPINB1 Antibody

ProSci
Product Code: PSI-22-088
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-088-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SERPINB1 antibody (22-088) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 60s.
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Immunofluorescence analysis of HeLa cells using SERPINB1 antibody (22-088). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SERPINB1 antibody (22-088) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using SERPINB1 antibody (22-088). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
SERPINB1, serpin peptidase inhibitor, clade B (ovalbumin), member 1, EI, ELANH2, LEI, M/NEI, MNEI, PI-2, PI2, leukocyte elastase inhibitor, peptidase inhibitor 2, protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived, serine (or cysteine) prot
Application Note:
WB: 1:500 - 1:2000

IF: 1:20 - 1:100
Background:
The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-379 of human SERPINB1 (NP_109591.1).
NCBI Gene ID #:
1992
NCBI Official Name:
serpin peptidase inhibitor, clade B (ovalbumin), member 1
NCBI Official Symbol:
SERPINB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 43kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P30740
User NOte:
Optimal dilutions for each application to be determined by the researcher.