CHAF1B Antibody

ProSci
Product Code: PSI-22-090
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-090-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CHAF1B antibody (22-090) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
2 / 5
Immunohistochemistry of paraffin-embedded rat fallopian tube using CHAF1B antibody (22-090) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human tonsil using CHAF1B antibody (22-090) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using CHAF1B antibody (22-090) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using CHAF1B antibody (22-090). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CHAF1B antibody (22-090) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat fallopian tube using CHAF1B antibody (22-090) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using CHAF1B antibody (22-090) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CHAF1B antibody (22-090) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using CHAF1B antibody (22-090). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CHAF1B, CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:20 - 1:100
Background:
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 270-559 of human CHAF1B (NP_005432.1).
NCBI Gene ID #:
8208
NCBI Official Name:
chromatin assembly factor 1, subunit B (p60)
NCBI Official Symbol:
CHAF1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13112
User NOte:
Optimal dilutions for each application to be determined by the researcher.