PGM1 Antibody

ProSci
Product Code: PSI-22-113
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-113-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PGM1 antibody (22-113) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunofluorescence analysis of U2OS cells using PGM1 antibody (22-113). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PGM1 antibody (22-113) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of U2OS cells using PGM1 antibody (22-113). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PGM1 (NP_002624.2).
NCBI Gene ID #:
5236
NCBI Official Name:
Phosphoglucomutase-1
NCBI Official Symbol:
PGM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 61kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P36871
User NOte:
Optimal dilutions for each application to be determined by the researcher.