RARS Antibody

ProSci
Product Code: PSI-22-116
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-116-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RARS antibody (22-116) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat brain using RARS antibody (22-116) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using RARS antibody (22-116) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using RARS antibody (22-116) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using RARS antibody (22-116). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RARS antibody (22-116) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using RARS antibody (22-116) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using RARS antibody (22-116) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RARS antibody (22-116) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RARS antibody (22-116). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
RARS, ArgRS, DALRD1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RARS (NP_002878.2).
NCBI Gene ID #:
5917
NCBI Official Name:
arginyl-tRNA synthetase
NCBI Official Symbol:
RARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P54136
User NOte:
Optimal dilutions for each application to be determined by the researcher.