DHRS9 Antibody

ProSci
Product Code: PSI-22-128
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-128-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DHRS9 antibody (22-128) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 390s.
2 / 5
Western blot analysis of extracts of various cell lines, using DHRS9 antibody (22-128) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat brain using DHRS9 Antibody (22-128) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using DHRS9 Antibody (22-128) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using DHRS9 antibody (22-128). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DHRS9 antibody (22-128) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 390s.
Western blot analysis of extracts of various cell lines, using DHRS9 antibody (22-128) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using DHRS9 Antibody (22-128) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DHRS9 Antibody (22-128) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DHRS9 antibody (22-128). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
3-alpha-HSD, 3ALPHA-HSD, RDH-E2, RDH-TBE, RDH15, RDHL, RDHTBE, RETSDR8, SDR9C4, dehydrogenase/reductase SDR family member 9, 3-alpha hydroxysteroid dehydrogenase, NADP-dependent retinol dehydrogenase/reductase, dehydrogenase/reductase (SDR family) member 9, retinol dehydrogenase 15, retinol dehydrogenase homolog, short chain dehydrogenase/reductase family 9C member 4, short-chain dehydrogenase/reductase retSDR8, tracheobronchial epithelial cell-specific retinol dehydrogenase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 18-319 of human DHRS9 (NP_001135743.1).
NCBI Gene ID #:
10170
NCBI Official Name:
dehydrogenase/reductase 9
NCBI Official Symbol:
DHRS9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
Q9BPW9
User NOte:
Optimal dilutions for each application to be determined by the researcher.