PCGF1 Antibody

Contact us
ProSci
Product Code: PSI-22-140
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-140-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using PCGF1 antibody (22-140).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of L-929 cells using PCGF1 antibody (22-140) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of U-2OS cells using PCGF1 antibody (22-140) at dilution of 1:100. Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using PCGF1 antibody (22-140).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of L-929 cells using PCGF1 antibody (22-140) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2OS cells using PCGF1 antibody (22-140) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
2010002K04Rik, NSPC1, RNF3A-2, RNF68, polycomb group RING finger protein 1, RING finger protein 68, nervous system Polycomb-1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-259 of human PCGF1 (NP_116062.2).
NCBI Gene ID #:
84759
NCBI Official Name:
polycomb group ring finger 1
NCBI Official Symbol:
PCGF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
Q9BSM1
User NOte:
Optimal dilutions for each application to be determined by the researcher.