ALPP Antibody

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ProSci
Product Code: PSI-22-150
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-150-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HeLa cells, using ALPP antibody (22-150) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug ALPP antibody (22-150). Western blot was performed from the immunoprecipitate using ALPP antibody (22-150) at a dilition of 1:1000.
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Western blot analysis of extracts of HeLa cells, using ALPP antibody (22-150) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug ALPP antibody (22-150). Western blot was performed from the immunoprecipitate using ALPP antibody (22-150) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
ALPP, ALP, PLAP, PALP, PLAP-1
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human ALPP (NP_001623.3).
NCBI Gene ID #:
250
NCBI Official Name:
alkaline phosphatase, placental
NCBI Official Symbol:
ALPP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Stem Cell
Swissprot #:
P05187
User NOte:
Optimal dilutions for each application to be determined by the researcher.