CLIC1 Antibody

ProSci
Product Code: PSI-22-159
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-159-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CLIC1 antibody (22-159) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat brain using CLIC1 antibody (22-159) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using CLIC1 antibody (22-159). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CLIC1 antibody (22-159) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CLIC1 antibody (22-159) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CLIC1 antibody (22-159). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CLIon Channel1, G6, NCC27
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-241 of human CLIC1 (NP_001279.2).
NCBI Gene ID #:
1192
NCBI Official Name:
chloride intracellular channel 1
NCBI Official Symbol:
CLIC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
O00299
User NOte:
Optimal dilutions for each application to be determined by the researcher.