MGAT1 Antibody

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ProSci
Product Code: PSI-22-178
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-22-178-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MGAT1 antibody (22-178) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver damage using MGAT1 antibody (22-178) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using MGAT1 antibody (22-178) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using MGAT1 antibody (22-178) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using MGAT1 antibody (22-178) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MGAT1 antibody (22-178) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
MGAT1, OTTHUMP00000161546, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase, MGAT, mannosyl (alpha-1, 3-)-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase, GNT-I, GNT-1, GLYT1, GLCT1, GLCNAC-TI, GGNT1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 156-445 of human MGAT1 (NP_001108092.1).
NCBI Gene ID #:
4245
NCBI Official Name:
mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
NCBI Official Symbol:
MGAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P26572
User NOte:
Optimal dilutions for each application to be determined by the researcher.