SERPINB9 Antibody

ProSci
Product Code: PSI-22-183
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-183-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SERPINB9 antibody (22-183) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of HeLa cells using SERPINB9 antibody (22-183). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SERPINB9 antibody (22-183) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using SERPINB9 antibody (22-183). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
SERPINB9, serpin peptidase inhibitor, clade B (ovalbumin), member 9, CAP-3, CAP3, PI9, protease inhibitor 9 (ovalbumin type), serine (or cysteine) proteinase inhibitor
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
This gene encodes a member of the serine protease inhibitor family which are also known as serpins. The encoded protein belongs to a subfamily of intracellular serpins. This protein inhibits the activity of the effector molecule granzyme B. Overexpression of this protein may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. A pseudogene of this gene is found on chromosome 6.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-376 of human SERPINB9 (NP_004146.1).
NCBI Gene ID #:
5272
NCBI Official Name:
serpin peptidase inhibitor, clade B (ovalbumin), member 9
NCBI Official Symbol:
SERPINB9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 42kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology
Swissprot #:
P50453
User NOte:
Optimal dilutions for each application to be determined by the researcher.