PYGB Antibody

ProSci
Product Code: PSI-22-192
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-192-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using PYGB antibody (22-192) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Immunofluorescence analysis of MCF-7 cells using PYGB antibody (22-192). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug PYGB antibody (22-192). Western blot was performed from the immunoprecipitate using PYGB antibody (22-192) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using PYGB antibody (22-192) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of MCF-7 cells using PYGB antibody (22-192). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug PYGB antibody (22-192). Western blot was performed from the immunoprecipitate using PYGB antibody (22-192) at a dilition of 1:1000.

Further Information

Additional Names:
PYGB, GPBB, Phosphorylase, glycogen, Phosphorylase, glycogen brain, Glycogen phosphorylase B
Application Note:
WB: 1:500 - 1:2000

IF: 1:20 - 1:100

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2).
NCBI Gene ID #:
5834
NCBI Official Name:
phosphorylase, glycogen; brain
NCBI Official Symbol:
PYGB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P11216
User NOte:
Optimal dilutions for each application to be determined by the researcher.