TRIM27 Antibody

ProSci
Product Code: PSI-22-195
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-195-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using TRIM27 antibody (22-195) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 4
Immunohistochemistry of paraffin-embedded human esophagus using TRIM27 Antibody (22-195) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse stomach using TRIM27 Antibody (22-195) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using TRIM27 antibody (22-195). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using TRIM27 antibody (22-195) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human esophagus using TRIM27 Antibody (22-195) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using TRIM27 Antibody (22-195) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TRIM27 antibody (22-195). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
TRIM27, RFP, RNF76
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 214-513 of human TRIM27 (NP_006501.1).
NCBI Gene ID #:
5987
NCBI Official Name:
tripartite motif containing 27
NCBI Official Symbol:
TRIM27
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 59kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P14373
User NOte:
Optimal dilutions for each application to be determined by the researcher.