SNRPA Antibody

ProSci
Product Code: PSI-22-200
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-200-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SNRPA antibody (22-200) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat liver using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
10 / 12
Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (18-626) and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Immunohistochemistry of paraffin-embedded mouse heart using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using SNRPA antibody (22-200).

Western blot analysis of extracts of various cell lines, using SNRPA antibody (22-200) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (18-626) and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunohistochemistry of paraffin-embedded mouse heart using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SNRPA antibody (22-200).

Further Information

Additional Names:
SNRPA, U1A, Mud1, U1-A
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human SNRPA (NP_004587.1).
NCBI Gene ID #:
6626
NCBI Official Name:
small nuclear ribonucleoprotein polypeptide A
NCBI Official Symbol:
SNRPA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 31kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P09012
User NOte:
Optimal dilutions for each application to be determined by the researcher.