SPINT1 Antibody

ProSci
Product Code: PSI-22-201
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-201-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SPINT1 antibody (22-201) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human prostate using SPINT1 antibody (22-201) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse esophageal cancer using SPINT1 antibody (22-201) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using SPINT1 antibody (22-201). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SPINT1 antibody (22-201) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using SPINT1 antibody (22-201) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse esophageal cancer using SPINT1 antibody (22-201) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SPINT1 antibody (22-201). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Kunitz-type protease inhibitor 1, Hepatocyte growth factor activator inhibitor type 1, HAI-1, SPINT1, HAI1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 234-513 of human SPINT1 (NP_003701.1).
NCBI Gene ID #:
6692
NCBI Official Name:
Kunitz-type protease inhibitor 1
NCBI Official Symbol:
SPINT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology
Swissprot #:
O43278
User NOte:
Optimal dilutions for each application to be determined by the researcher.