AAAS Antibody

ProSci
Product Code: PSI-22-213
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-213-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using AAAS antibody (22-213) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat brain using AAAS Antibody (22-213) at dilution of 1:100 (20x lens).
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Immunohistochemistry of paraffin-embedded human appendicitis using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using AAAS antibody (22-213). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using AAAS antibody (22-213) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using AAAS Antibody (22-213) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded human appendicitis using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AAAS antibody (22-213). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Aladin, Adracalin, AAAS, ADRACALA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the WD-repeat family of regulatory proteins and may be involved in normal development of the peripheral and central nervous system. The encoded protein is part of the nuclear pore complex and is anchored there by NDC1. Defects in this gene are a cause of achalasia-addisonianism-alacrima syndrome (AAAS), also called triple-A syndrome or Allgrove syndrome. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 287-546 of human AAAS (NP_056480.1).
NCBI Gene ID #:
8086
NCBI Official Name:
Aladin
NCBI Official Symbol:
AAAS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 59kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q9NRG9
User NOte:
Optimal dilutions for each application to be determined by the researcher.