H6PD Antibody

ProSci
Product Code: PSI-22-223
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-223-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using H6PD Antibody (22-223) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using H6PD antibody (22-223) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U2OS cells using H6PD antibody (22-223). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using H6PD Antibody (22-223) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using H6PD antibody (22-223) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using H6PD antibody (22-223). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
H6PD, DKFZp686A01246, G6PDH, GDH, MGC87643, CORTRD1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human H6PD (NP_004276.2).
NCBI Gene ID #:
9563
NCBI Official Name:
hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
NCBI Official Symbol:
H6PD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 89kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O95479
User NOte:
Optimal dilutions for each application to be determined by the researcher.