DHRS2 Antibody

ProSci
Product Code: PSI-22-229
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-229-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DHRS2 antibody (22-229) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human breast cancer using DHRS2 antibody (22-229) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human uterine cancer using DHRS2 antibody (22-229) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using DHRS2 antibody (22-229) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of HeLa cells using DHRS2 antibody (22-229). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DHRS2 antibody (22-229) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using DHRS2 antibody (22-229) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using DHRS2 antibody (22-229) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DHRS2 antibody (22-229) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DHRS2 antibody (22-229). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DHRS2, HEP27, SDR25C1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family, which has over 46,000 members. Members of this family are enzymes that metabolize many different compounds, such as steroid hormones, prostaglandins, retinoids, lipids and xenobiotics. Alternative promoter use and alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DHRS2 (NP_878912.1).
NCBI Gene ID #:
10202
NCBI Official Name:
dehydrogenase/reductase (SDR family) member 2
NCBI Official Symbol:
DHRS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30-35kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
Q13268
User NOte:
Optimal dilutions for each application to be determined by the researcher.