DIDO1 Antibody

ProSci
Product Code: PSI-22-235
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-235-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunofluorescence analysis of HeLa cells using DIDO1 antibody (22-235). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using DIDO1 antibody (22-235) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Immunofluorescence analysis of HeLa cells using DIDO1 antibody (22-235). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using DIDO1 antibody (22-235) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Further Information

Additional Names:
DIDO1, BYE1, DIO1, DATF1, DIDO2, DIDO3, DIO-1, DATF-1, C20orf158, dJ885L7.8
Application Note:
WB: 1:500 - 1:2000

IF: 1:20 - 1:50
Background:
Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DIDO1 (NP_071388.2).
NCBI Gene ID #:
11083
NCBI Official Name:
death inducer-obliterator 1
NCBI Official Symbol:
DIDO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 76kDa/244kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
Q9BTC0
User NOte:
Optimal dilutions for each application to be determined by the researcher.