DDAH2 Antibody

ProSci
Product Code: PSI-22-238
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-238-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DDAH2 antibody (22-238) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 2
Immunofluorescence analysis of U2OS cells using DDAH2 antibody (22-238). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DDAH2 antibody (22-238) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using DDAH2 antibody (22-238). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DDAH2, DDAH, DDAHII, G6a, NG30, HEL-S-277
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:10 - 1:100
Background:
This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH2 (NP_039268.1).
NCBI Gene ID #:
23564
NCBI Official Name:
dimethylarginine dimethylaminohydrolase 2
NCBI Official Symbol:
DDAH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience
Swissprot #:
O95865
User NOte:
Optimal dilutions for each application to be determined by the researcher.