GNL3 Antibody

ProSci
Product Code: PSI-22-239
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-239-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of 293T cells, using GNL3 antibody (22-239) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunofluorescence analysis of MCF7 cells using GNL3 antibody (22-239). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug GNL3 antibody (22-239). Western blot was performed from the immunoprecipitate using GNL3 antibody (22-239) at a dilition of 1:500.

Western blot analysis of extracts of 293T cells, using GNL3 antibody (22-239) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of MCF7 cells using GNL3 antibody (22-239). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug GNL3 antibody (22-239). Western blot was performed from the immunoprecipitate using GNL3 antibody (22-239) at a dilition of 1:500.

Further Information

Additional Names:
GNL3, guanine nucleotide binding protein-like 3 (nucleolar), C77032, E2IG3, NNP47, NS, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide-binding protein-like 3, novel nucleolar protein 47, nucleolar GTP-binding pr
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100

IP: 1:50 - 1:100
Background:
The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GNL3 (NP_996562.1).
NCBI Gene ID #:
26354
NCBI Official Name:
guanine nucleotide binding protein-like 3 (nucleolar)
NCBI Official Symbol:
GNL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 62kDa
Purification:
Affinity purification
Research Area:
Stem Cell
Swissprot #:
Q9BVP2
User NOte:
Optimal dilutions for each application to be determined by the researcher.