AHSP Antibody

ProSci
Product Code: PSI-22-245
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-245-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using AHSP antibody (22-245) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded rat testis using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using AHSP antibody (22-245) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using AHSP antibody (22-245) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat testis using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using AHSP antibody (22-245) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
EDRF, ERAF, alpha-hemoglobin-stabilizing protein, alpha hemoglobin stabilising protein, erythroid differentiation associated factor, erythroid differentiation-related factor
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a molecular chaperone which binds specifically to free alpha-globin and is involved in hemoglobin assembly. The encoded protein binds to monomeric alpha-globin until it has been transferred to beta-globin to form a heterodimer, which in turn binds to another heterodimer to form the stable tetrameric hemoglobin. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human AHSP (NP_057717.1).
NCBI Gene ID #:
51327
NCBI Official Name:
alpha hemoglobin stabilizing protein
NCBI Official Symbol:
AHSP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 12kDa
Purification:
Affinity purification
Research Area:
Signal Transduction, Stem Cell
Swissprot #:
Q9NZD4
User NOte:
Optimal dilutions for each application to be determined by the researcher.