UCN2 Antibody

ProSci
Product Code: PSI-22-258
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-258-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of rat skin, using UCN2 antibody (22-258) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse heart using UCN2 antibody (22-258) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using UCN2 antibody (22-258). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of rat skin, using UCN2 antibody (22-258) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse heart using UCN2 antibody (22-258) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using UCN2 antibody (22-258). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
SRP, UCN-II, UCNI, UR, URP, urocortin-2, prepro-urocortin 2, stresscopin-related peptide, ucn II, urocortin II, urocortin-related peptide
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-112 of human UCN2 (NP_149976.1).
NCBI Gene ID #:
90226
NCBI Official Name:
urocortin 2
NCBI Official Symbol:
UCN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 12kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q96RP3
User NOte:
Optimal dilutions for each application to be determined by the researcher.