DDIT3 / CHOP Antibody

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ProSci
Product Code: PSI-22-272
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-272-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DDIT3 / CHOP antibody (22-272) at 1:1000 dilution. Both C2C12 cells and C6 cells were treated by tunicamycin (2 ug/ml) for 8 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 3min.
2 / 2
Immunofluorescence analysis of A549 cells using DDIT3 / CHOP antibody (22-272). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using DDIT3 / CHOP antibody (22-272) at 1:1000 dilution. Both C2C12 cells and C6 cells were treated by tunicamycin (2 ug/ml) for 8 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 3min.
Immunofluorescence analysis of A549 cells using DDIT3 / CHOP antibody (22-272). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
DNA damage-inducible transcript 3 protein, DDIT-3, C/EBP zeta, C/EBP-homologous protein, CHOP, C/EBP-homologous protein 10, CHOP-10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible protein GADD153, DDIT3, CHOP, CHOP10, GADD153
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human DDIT3 / CHOP
NCBI Gene ID #:
1649
NCBI Official Name:
DNA damage-inducible transcript 3 protein
NCBI Official Symbol:
DDIT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Autophagy, Cell Cycle, Obesity, Signal Transduction, Stem Cell, Transcription
Swissprot #:
P35638
User NOte:
Optimal dilutions for each application to be determined by the researcher.