HPD Antibody

ProSci
Product Code: PSI-22-273
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-273-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HPD antibody (22-273) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat kidney using HPD antibody (22-273) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using HPD antibody (22-273) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using HPD antibody (22-273). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HPD antibody (22-273) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat kidney using HPD antibody (22-273) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HPD antibody (22-273) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HPD antibody (22-273). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, HPD, PPD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human HPD (NP_002141.1).
NCBI Gene ID #:
3242
NCBI Official Name:
4-hydroxyphenylpyruvate dioxygenase
NCBI Official Symbol:
HPD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
P32754
User NOte:
Optimal dilutions for each application to be determined by the researcher.