AGPAT2 Antibody

ProSci
Product Code: PSI-22-280
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-280-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HT-29 cells, using AGPAT2 antibody (22-280) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunofluorescence analysis of HeLa cells using AGPAT2 antibody (22-280). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of HT-29 cells, using AGPAT2 antibody (22-280) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using AGPAT2 antibody (22-280). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
AGPAT2, 1-AGPAT2, BSCL, BSCL1, LPAAB, LPAAT-beta
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 199-278 of human AGPAT2 (NP_006403.2).
NCBI Gene ID #:
10555
NCBI Official Name:
1-acylglycerol-3-phosphate O-acyltransferase 2
NCBI Official Symbol:
AGPAT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 31kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
O15120
User NOte:
Optimal dilutions for each application to be determined by the researcher.