ATOH1 Antibody

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ProSci
Product Code: PSI-22-289
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-289-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATOH1 Antibody (22-289) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunofluorescence analysis of MCF7 cells using ATOH1 antibody (22-289). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ATOH1 Antibody (22-289) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of MCF7 cells using ATOH1 antibody (22-289). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ATOH1, ATH1, HATH1, MATH-1, bHLHa14
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human ATOH1 (NP_005163.1).
NCBI Gene ID #:
474
NCBI Official Name:
atonal homolog 1 (Drosophila)
NCBI Official Symbol:
ATOH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience, Stem Cell, Transcription
Swissprot #:
Q92858
User NOte:
Optimal dilutions for each application to be determined by the researcher.