BLMH Antibody

ProSci
Product Code: PSI-22-293
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-293-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using BLMH Antibody (22-293) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunofluorescence analysis of HeLa cells using BLMH antibody (22-293). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using BLMH Antibody (22-293) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using BLMH antibody (22-293). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:20 - 1:50
Background:
Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BLMH (NP_000377.1).
NCBI Gene ID #:
642
NCBI Official Name:
Bleomycin hydrolase
NCBI Official Symbol:
BLMH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
Q13867
User NOte:
Optimal dilutions for each application to be determined by the researcher.