CARS Antibody

ProSci
Product Code: PSI-22-297
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-297-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CARS antibody (22-297) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 3
Immunohistochemistry of paraffin-embedded mouse heart using CARS antibody (22-297) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of HeLa cells using CARS antibody (22-297). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CARS antibody (22-297) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded mouse heart using CARS antibody (22-297) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CARS antibody (22-297). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CARS, CARS1, CYSRS, MGC:11246
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 469-748 of human CARS (NP_001742.1).
NCBI Gene ID #:
833
NCBI Official Name:
cysteinyl-tRNA synthetase
NCBI Official Symbol:
CARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 85kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P49589
User NOte:
Optimal dilutions for each application to be determined by the researcher.