CCT2 Antibody

ProSci
Product Code: PSI-22-302
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-302-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using CCT2 antibody (22-302) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 3
Immunofluorescence analysis of HeLa cells using CCT2 antibody (22-302). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug CCT2 antibody (22-302). Western blot was performed from the immunoprecipitate using CCT2 antibody (22-302) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using CCT2 antibody (22-302) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunofluorescence analysis of HeLa cells using CCT2 antibody (22-302). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug CCT2 antibody (22-302). Western blot was performed from the immunoprecipitate using CCT2 antibody (22-302) at a dilition of 1:1000.

Further Information

Additional Names:
CCT2, 99D8.1, CCT-beta, CCTB, PRO1633, TCP-1-beta, TCP1 Beta
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 206-535 of human CCT2 (NP_006422.1).
NCBI Gene ID #:
10576
NCBI Official Name:
chaperonin containing TCP1, subunit 2 (beta)
NCBI Official Symbol:
CCT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P78371
User NOte:
Optimal dilutions for each application to be determined by the researcher.