CMPK1 Antibody

ProSci
Product Code: PSI-22-313
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-313-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CMPK1 antibody (22-313) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of MCF7 cells using CMPK1 antibody (22-313). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CMPK1 antibody (22-313) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of MCF7 cells using CMPK1 antibody (22-313). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CMPK1, CMPK, Deoxycytidylate kinase, Cytidine monophosphate kinase, RP11-511I2.1, UMP-CMPK, UMP/CMP kinase, Uridine monophosphate kinase, UCK, UMK, UMP-CMP kinase, UMP/CMPK, CMK, Cytidylate kinase, UMPK
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 69-228 of human CMPK1 (NP_057392.1).
NCBI Gene ID #:
51727
NCBI Official Name:
cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
NCBI Official Symbol:
CMPK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 22kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P30085
User NOte:
Optimal dilutions for each application to be determined by the researcher.