CPM Antibody

ProSci
Product Code: PSI-22-316
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-316-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HeLa cells, using CPM Antibody (22-316) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded human stomach using CPM antibody (22-316) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver damage using CPM antibody (22-316) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using CPM antibody (22-316) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of HeLa cells, using CPM Antibody (22-316) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human stomach using CPM antibody (22-316) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using CPM antibody (22-316) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using CPM antibody (22-316) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Carboxypeptidase M, CPM, CPM
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 204-443 of human CPM (NP_001005502.1).
NCBI Gene ID #:
1368
NCBI Official Name:
Carboxypeptidase M
NCBI Official Symbol:
CPM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 62kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Immunology, Innate Immunity, Signal Transduction
Swissprot #:
P14384
User NOte:
Optimal dilutions for each application to be determined by the researcher.