CRHBP Antibody

ProSci
Product Code: PSI-22-319
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-319-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CRHBP antibody (22-319) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded rat heart using CRHBP antibody (22-319) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human prostate using CRHBP antibody (22-319) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using CRHBP antibody (22-319) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of HeLa cells using CRHBP antibody (22-319). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CRHBP antibody (22-319) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using CRHBP antibody (22-319) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using CRHBP antibody (22-319) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CRHBP antibody (22-319) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CRHBP antibody (22-319). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CRF-BP, CRFBP, corticotropin-releasing factor-binding protein, CRF-binding protein, CRH-BP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-322 of human CRHBP (NP_001873.2).
NCBI Gene ID #:
1393
NCBI Official Name:
corticotropin releasing hormone binding protein
NCBI Official Symbol:
CRHBP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P24387
User NOte:
Optimal dilutions for each application to be determined by the researcher.