CSRP3 Antibody

ProSci
Product Code: PSI-22-320
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-320-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CSRP3 antibody (22-320) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CSRP3 antibody (22-320) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CSRP3, CLP, MLP, CRP3, LMO4, CMD1M, CMH12
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human CSRP3 (NP_003467.1).
NCBI Gene ID #:
8048
NCBI Official Name:
cysteine and glycine-rich protein 3 (cardiac LIM protein)
NCBI Official Symbol:
CSRP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
P50461
User NOte:
Optimal dilutions for each application to be determined by the researcher.